SANTA CRUZ:Vimentin Antibody (V9): sc-6260

• Vimentin Antibody (V9) is a mouse monoclonal IgG1 κ Vimentin antibody, cited in 1,158 publications, provided at 200 µg/ml

• raised against purified Vimentin from porcine eye lens

• Vimentin Antibody (V9) is recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including porcine

• Anti-Vimentin Antibody (V9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM

• also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems

• also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems

• also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM

• m-IgGκ BP-HRP is the preferred secondary detection reagent for Vimentin Antibody (V9) for WB and IHC(P) applications. This reagent is now offered in a bundle with Vimentin Antibody (V9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

維門丁抗體（V9）是一種針對(duì)豬眼晶體純化的維門蛋白（主要表達(dá)于間充質(zhì)細(xì)胞中的關(guān)鍵細(xì)胞骨架蛋白）培養(yǎng)的小鼠單克隆IgG1 κ輕鏈抗體。Vimentin小鼠單克隆抗體（V9）識(shí)別Vimentin免疫原序列中的表位，并在西部吸跡（WB）、免疫沉淀（IP）、免疫熒光（IF）、石蠟嵌入切片的免疫組織化學(xué)（IHCP）和流式細(xì)胞術(shù)（FCM）應(yīng)用中表現(xiàn)出廣泛的物種交叉反應(yīng)，包括小鼠、大鼠和人類樣本。抗維門素單克隆抗體（V9）有非偶聯(lián)形式和多種結(jié)合形式，包括辣根過(guò)氧化物酶、藍(lán)藻酯、熒光素異硫氰酸酯、瓊脂糖和各種Alexa氟®偶聯(lián)物，具備多樣化的實(shí)驗(yàn)需求。維曼素屬于中間絲家族，能夠保持細(xì)胞結(jié)構(gòu)、完整性和機(jī)械韌性。維曼蛋白與細(xì)胞角蛋白、肌動(dòng)蛋白和微管等多種蛋白質(zhì)相互作用，形成復(fù)雜的細(xì)胞骨架纖維網(wǎng)絡(luò)。維曼蛋白與運(yùn)動(dòng)蛋白的結(jié)合促進(jìn)了細(xì)胞內(nèi)運(yùn)輸和細(xì)胞器定位。雖然主要存在于細(xì)胞質(zhì)內(nèi)，但維氏蛋白在特定條件下會(huì)定位于細(xì)胞核和細(xì)胞膜。維門素是上皮向間充質(zhì)過(guò)渡（EMT）的關(guān)鍵標(biāo)志物，EMT是傷口愈合、發(fā)育和腫瘤轉(zhuǎn)移的關(guān)鍵過(guò)程。維門素的表達(dá)模式在癌癥生物學(xué)中尤為重要，因?yàn)榫S曼素在多種腫瘤中常表現(xiàn)出上調(diào)，包括黑色素瘤、肉瘤和乳腺癌，維曼素常與細(xì)胞角蛋白及其他中間絲共表達(dá)。Vimentin（V9）抗體使研究人員能夠通過(guò)Vimentin在細(xì)胞質(zhì)和膜染色的一致性特性，研究細(xì)胞骨架動(dòng)力學(xué)、癌癥進(jìn)展及組織特異性表達(dá)模式。

Santa Cruz Biotechnology（簡(jiǎn)稱 Santa Cruz，圣克魯斯）是生物醫(yī)學(xué)研究市場(chǎng)的核心參與者，總部位于美國(guó)德克薩斯州，自 1996 年成立以來(lái)，三十余年間始終專注于單克隆抗體、生物化學(xué)試劑、實(shí)驗(yàn)室器具及 CRISPR 基因編輯產(chǎn)品的研發(fā)與生產(chǎn)。

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SANTA CRUZ:Vimentin Antibody (V9): sc-6260